Microfluidic cryofixation for correlative microscopy.

Mejia, Y. X.; Feindt, H.; Zhang, D.; Steltenkamp, S.; Burg, T. P.

doi:10.1039/c4lc00333k

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Journal Article

Microfluidic cryofixation for correlative microscopy.

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Mejia, Y. X.
Research Group of Biological Micro- and Nanotechnology, MPI for biophysical chemistry, Max Planck Society;

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Burg, T. P.
Research Group of Biological Micro- and Nanotechnology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Mejia, Y. X., Feindt, H., Zhang, D., Steltenkamp, S., & Burg, T. P. (2014). Microfluidic cryofixation for correlative microscopy. Lab on a Chip, 14(17), 3281-3284. doi:10.1039/c4lc00333k.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-ED3F-1

Abstract

Cryofixation yields outstanding ultrastructural preservation of cells for electron microscopy, but current methods disrupt live cell imaging. Here we demonstrate a microfluidic approach that enables cryofixation to be performed directly in the light microscope with millisecond time resolution and at atmospheric pressure. This will provide a link between imaging/stimulation of live cells and post-fixation optical, electron, or X-ray microscopy.