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Journal Article

Gene expression in Acetabularia. II. Analysis of in vitro translation products

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Shoeman, Robert L.
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Shoeman, R. L., & Schweiger, H. (1982). Gene expression in Acetabularia. II. Analysis of in vitro translation products. Journal of Cell Science, 58(1), 35-48. Retrieved from http://jcs.biologists.org/cgi/content/abstract/58/1/35.

Abstract

The translation products induced by poly(A)+ RNA from Acetabularia mediterranea, A. cliftonii and A. ryukyuensis in a modified, highly efficient wheat germ cell−free in vitro system were separated by two−dimensional gel electrophoresis. A comparison of the translation products on the basis of their molecular weight and their isoelectric point revealed only a limited similarity between the patterns of the three species. The pronounced species specificity will permit the study of the in vivo translation of heterologous poly(A)+ RNA in Acetabularia cytoplasm