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Journal Article

Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins

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Scherbarth, Annemarie
Light Microscopy Facility, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Giese, Günter
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;
Light Microscopy Facility, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Paulin−Levasseur, M., Scherbarth, A., Giese, G., Roser, K., Bohn, W., & Traub, P. (1989). Expression of nuclear lamins in mammalian somatic cells lacking cytoplasmic intermediate filament proteins. Journal of Cell Science, 92(3), 361-370. Retrieved from http://jcs.biologists.org/cgi/content/abstract/92/3/361?maxtoshow%3D%26HITS%3D10%26hits%3D10%26RESULTFORMAT%3D%26andorexacttitle%3Dand%26andorexacttitleabs%3Dand%26andorexactfulltext%3Dand%26searchid%3D1%26FIRSTINDEX%3D0%26sortspec%3Drelevance%26volume%3D92%26firstpage%3D361%26resourcetype%3DHWCIT.

Abstract

Using immunofluorescence and immunoblotting techniques, we have examined the composition of the nuclear lamina in murine plasmacytoma cells, MPC−11, exposed to the phorbol ester TPA as well as in two cell lines devoid of cytoplasmic intermediate filament proteins, the human adrenal cortex carcinoma−derived cells SW−13 and the clone C6−M−D4 derived from the rat glial cell line C6. Our results show that the inhibition of proliferation and the induction of vimentin synthesis observed in TPA−treated MPC−11 populations are not paralleled by changes in the lamin complement of these cells, which contain lamin B but lack lamins A and C. Furthermore, the analysis performed on SW−13 and C6−M−D4 cell lines clearly demonstrates that mammalian somatic cells display considerable variations in lamin expression and indicates that lamin B may be the only lamin species constitutively expressed in mammalian cells