RNA editing of AMPA receptor subunit GluR-B: A base-paired intron-exon 
structure determines position and efficiency

Higuchi, Miyoko; Single, Frank Nicolai; Köhler, Martin; Sommer, Bernd; Sprengel, Rolf; Seeburg, Peter H.

doi:10.1016/0092-8674(93)90622-W

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RNA editing of AMPA receptor subunit GluR-B: A base-paired intron-exon structure determines position and efficiency

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Higuchi, Miyoko
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Single, Frank Nicolai
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Köhler, Martin
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Sprengel, Rolf
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society;
Olfaction Web, Max Planck Institute for Medical Research, Max Planck Society;

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Seeburg, Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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http://www.sciencedirect.com/science/article/pii/009286749390622W/pdf?md5=226bc99acdaf0ef05b1c34acad2b89a6&pid=1-s2.0-009286749390622W-main.pdf
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https://doi.org/10.1016/0092-8674(93)90622-W
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Citation

Higuchi, M., Single, F. N., Köhler, M., Sommer, B., Sprengel, R., & Seeburg, P. H. (1993). RNA editing of AMPA receptor subunit GluR-B: A base-paired intron-exon structure determines position and efficiency. Cell, 75(7), 1361-1370. doi:10.1016/0092-8674(93)90622-W.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-A9E6-F

Abstract

A functionally critical position (Q/R site) of the AMPA receptor subunit GluR-B is controlled by RNA editing that operates in the nucleus, since in brain and clonal cell lines of neural origin, unspliced GluR-B transcripts occur edited in the Q/R site CAG codon and, additionally, in intronic adenosines. Transfection of GluR-B gene constructs into PC12 cells revealed that the proximal part of the intron downstream of the unedited exonic site is required for Q/R site editing. This intron portion contains an imperfect inverted repeat preceding a 10 nt sequence with exact complementarity to the exon centered on the unedited codon. Single nucleotide substitutions in this short intronic sequence or its exonic complement curtailed Q/R site editing, which was recovered by restoring complementarity in the respective partner strand. Base conversion in the channel-coding region of GluR-B directed by base paired sequences may be executed by a ubiquitous nuclear adenosine deaminase specific for double-stranded RNA.