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Journal Article

PVP-containing solutions for analysis of divalent cation-dependent NMDA responses in Xenopus oocytes

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/persons/resource/persons94867

Raditsch,  Martin
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95970

Witzemann,  Veit
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Working Group Witzemann / Koenen, Max Planck Institute for Medical Research, Max Planck Society;
Molecular anatomy of the neuromuscular junction, Max Planck Institute for Medical Research, Max Planck Society;
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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http://onlinelibrary.wiley.com/doi/10.1016/0014-5793(94)01118-4/epdf
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https://doi.org/10.1016/0014-5793(94)01118-4
(Any fulltext)

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Citation

Raditsch, M., & Witzemann, V. (1994). PVP-containing solutions for analysis of divalent cation-dependent NMDA responses in Xenopus oocytes. FEBS Letters, 354(2), 177-182. doi:10.1016/0014-5793(94)01118-4.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-A8AE-6
Abstract
The electrophysiological analysis of Ca(2+)-conducting ion channels in Xenopus oocytes is difficult due to secondary intracellular effects induced by Ca2+. In the presence of polyvinylpyrrolidone (PVP) membrane currents can be recorded in nominally divalent cation-free solutions. The Ca(2+)-permeable recombinant NMDA receptors of the NR1/NR2A subtype were used as assay system and the results show that PVP has no effect on NMDA receptor-induced currents. Ca2+ and Ba2+ depress NMDA-induced currents at submillimolar concentrations probably by interfering with the Na+/K+ flux. This block is fully reversible as also observed for Mg2+ but shows in contrast no pronounced voltage dependence. PVP-containing solutions may be useful for the analysis of divalent cation-dependent ion channels.