English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONS
  This item is discarded!Release HistoryDetailsSummary

Discarded
Journal Article

A holidic medium for Drosophila melanogaster

MPS-Authors
/persons/resource/persons129943

Hoddinott,  Matthew P.
external;
Biological Mechanisms of Ageing, Department Partridge, Max Planck Institute for Biology of Ageing, Max Planck Society;

/persons/resource/persons129430

Hopfen,  Corinna
Biological Mechanisms of Ageing, Department Partridge, Max Planck Institute for Biology of Ageing, Max Planck Society;

/persons/resource/persons129875

Soultoukis,  Georgios
Biological Mechanisms of Ageing, Department Partridge, Max Planck Institute for Biology of Ageing, Max Planck Society;

/persons/resource/persons129348

Partridge,  Linda
Biological Mechanisms of Ageing, Department Partridge, Max Planck Institute for Biology of Ageing, Max Planck Society;

External Resource

(No access)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Piper, M. D. W., Blanc, E., Leitao-Goncalves, R., Yang, M., He, X., Linford, N. J., et al. (2014). A holidic medium for Drosophila melanogaster. NATURE METHODS, 11(1), 100-105. doi:10.1038/NMETH.2731.


Abstract
A critical requirement for research using model organisms is a well-defined and consistent diet. There is currently no complete chemically defined (holidic) diet available for Drosophila melanogaster. We describe a holidic medium that is equal in performance to an oligidic diet optimized for adult fecundity and lifespan. This holidic diet supports development over multiple generations but at a reduced rate. Over 7 years of experiments, the holidic diet yielded more consistent experimental outcomes than did oligidic food for egg laying by females. Nutrients and drugs were more available to flies in holidic medium and, similar to dietary restriction on oligidic food, amino acid dilution increased fly lifespan. We used this holidic medium to investigate amino acid-specific effects on food-choice behavior and report that folic acid from the microbiota is sufficient for Drosophila development.