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[(.o)19.6(rg)20.2(/a)16.5(ct)19.2(io)19(n/)18.9(ma)21.9(rk)20.2(er)18.4(/v)15.2(i)15.4(e)0(w)17.4(/)0(Z)18.1(D)0(B)20.9(-)0(G)19.8(E)0(N)16(E)0(-)21.8(0)0(0)19.9(0)0(4)19.9(2)0(6)19.9(-)0(1)19(\))-177.5(\()73.5(g)19(e)12.7(n)0(e)-167.7(s)0(u)16.3(m)0(-)]TJ
T*
[(ma)15.6(ry)20.2()67.7()-292.7(option\);)-308.5(second,)-303.4(gene)-311.1(expression)-307.2(patterns)-304.3(of)-306.7(the)-300.9(same)]TJ
0 -1.2266 TD
[(gen)11.3(es)-184.6(are)-189.8(an)16.1(not)12.3(at)12.7(ed)-192.6(wi)13.8(th)-187.2(cur)11.3(at)12.7(ed)-186.3(sta)11.3(ge)14.7(s)-197.3(w)0(h)14(e)0(n)-193(the)15.2(ir)-187.6(exp)15.6(re)12(ssi)12.8(on)]TJ
0 -1.2203 TD
[(was)-166.8(obs)16.8(er)12.1(ve)12(d)-173.7(\(se)17.3(e)-176.9(htt)14.6(p:/)]TJ
10.1736 0 TD
[(/z)13.5(n.)12.7(org)17.4(/d)13(at)12.7(a_t)13.2(ra)12.4(ns)14.3(fer)11.6(/D)14.6(own)17.5(loa)13.1(ds)14.7(/)]TJ
-10.1736 -1.2203 TD
[(wildtype-expression.txt\))-313(\()54.6(wild-type)-304.7(expression)45()-299.1(option\).)-302.2(As)]TJ
0 -1.2266 TD
[(genes)-476.8(can)-476.4(be)-472.2(expressed)-483.8(in)-474.8(different)-479.7(anatomical)-470.7(structures,)]TJ
0 -1.2203 TD
[(depending)-274.8(on)-265.4(the)-269.3(developmental)-268.5(stage,)-267.8(it)-263.2(is)-270.4(crucial)-266.4(to)-268.8(extract)]TJ
T*
[(the)-300.9(stage)-290.1(information)-302.6(for)-294.8(any)-291.8(kind)-292.5(of)-294.1(analysis.)-297.7(For)-292.4(both)-295.6(data)]TJ
T*
[(source)-321.5(options,)-322(this)-324.8(information)-321.6(can)-318.3(be)-314.1(extracted)-321.5(either)-324(by)-318.7(a)]TJ
0 -1.2266 TD
[(direct)-361(link)-362.6(between)-363.7(anatomical)-363.2(terms)-360.4(and)-360(stage)-359.7(of)-357.3(develop-)]TJ
0 -1.2203 TD
[(ment)-437.6(\(see)-437.9(http:/)]TJ
7.1196 0 TD
(/zn.org/data_transfer/Downloads/staged)Tj
-7.1196 -1.2203 TD
[(_anatomy.txt\),)-212.6(or)-205.8(directly)-204.5(from)-210.2(the)-206.1(experimental)-207.9(data)-204.8(records,)]TJ
0 -1.2266 TD
[(although)-322.3(data)-312.3(are)-316.3(not)-316.5(always)-313.4(available)-317.7(for)-313.8(each)-318.2(stage.)-312(Thus,)]TJ
0 -1.2203 TD
[(when)-198(the)-193.4()55(gene)-192.9(summary)52.6()-191.6(option)-197.7(is)-188.2(chosen,)-196.3(anatomical)-198.8(terms)]TJ
T*
[(are)-411.1(restricted)-413.9(based)-406.5(on)-410.8(their)-412.7(existence)-414.5(at)-404.6(the)-408.4(selected)-412(stage)]TJ
0 -1.2266 TD
[(\(Fig.)-243.4(1A\),)-238.2(while)-239.6(with)-243(the)-237.7()55(gene)-243.4(summary)52.6()-235.8(option,)-244.8(anatomical)]TJ
0 -1.2203 TD
[(terms)-404.6(are)-398.5(selected)-405.6(if)-402.3(the)-402.1(user-dened)-401.6(stage)-404(matches)-406.1(one)-398.1(of)]TJ
T*
[(their)-286.2(curated)-282.4(stages)-285.1(\(Fig.)-275(1B\).)]TJ
/F9 1 Tf
0 -2.447 TD
[(The)-337(ZEOGS)-336.3(software)]TJ
/F4 1 Tf
1.1128 -1.8336 TD
[(ZE)18.1(OG)17.2(S)-188.2(i)0(s)-181.9(a)-189.2(we)17.4(b-)20.4(ba)21.4(se)17.8(d)-192.7(p)0(r)16.9(o)0(g)24.4(r)0(a)18.8(m)-193.6(im)24.2(pl)14.1(e)12.7(m)0(e)15.2(n)0(t)21.4(i)0(n)18.4(g)-187.8(an)-186.3(a)13.1(l)0(g)17.3(o)0(r)21.8(i)0(t)15(h)0(m)]TJ
-1.1128 -1.2203 TD
[(wr)16.6(it)21.3(te)18.6(n)-521.9(i)0(n)-519.1(P)0(e)24(r)0(l)14.7(/)0(C)22.1(G)0(I)18.1(,)-521.4(wh)20.3(ic)16.2(h)-521.9(s)0(u)22.6(m)0(m)17.5(a)13.1(ri)14.7(z)13.1(e)0(s)-513.4(t)0(h)15.1(e)-518.4(mo)18.6(st)-513.9(ov)15.5(e)12.8(r)0(-)17.8(r)0(e)18.4(-)]TJ
T*
[(pr)16.9(es)17.8(e)12.8(n)0(t)15.1(e)12.8(d)-470.9(a)13.1(na)16.1(t)12.2(o)0(m)18.6(i)0(c)16.2(a)13.1(l)-465.1(t)0(e)18.6(r)0(m)20.7(s)-469.2(in)-455.8(an)-458.1(in)18.4(pu)16.3(t)-462(g)0(e)14.7(n)0(e)-458.5(s)0(e)24.1(t)-468.4(an)16.1(d)-464.6(t)0(h)15.1(e)12.7(ir)]TJ
0 -1.2266 TD
[(st)17.2(at)19(is)20.4(ti)15(c)13.4(a)0(l)-268.6(s)0(i)20.4(g)0(n)17.5(i)15.4(c)16.7(a)13.1(n)0(c)16.3(e)0(.)-268.4(F)14.6(ig)17.3(ur)16.9(e)-271.8(2)-271.4(il)18.2(lu)20.4(st)17.2(ra)18.8(te)18.6(s)-273.2(t)0(h)15.1(e)-271.8(wo)14.6(rk)20.2(o)19.5(w)-273.5(o)0(f)-262.5(t)0(h)15.1(e)]TJ
0 -1.2203 TD
[(pr)16.9(og)18.1(ra)18.8(m)15.1(.)-300.1(As)-287.2(in)18.4(pu)16.3(t)12.2(,)-300.1(t)12.2(h)0(e)-294.1(u)0(s)22.6(e)0(r)-291.4(p)0(r)23.2(o)0(v)15.5(i)0(d)18.8(e)12.8(s)-298.5(a)-296.7(li)18.2(st)-292.6(of)-287.8(ge)21(ne)-287.8(id)18.8(en)15.7(t)12.2(i)0()18.8(e)0(r)18.4(s)]TJ
T*
[(\(e)18.6(it)15(he)22(r)-285.2(g)0(e)21(n)0(e)-281.5(s)0(y)19.6(m)15.1(bo)18.1(ls)-276.7(of)-275.1(ZF)16.6(IN)-274(ID)21.8(s\))-273.7(an)16.1(d)-281.2(s)0(e)17.8(l)0(e)15.5(c)13.4(ts)-280(t)12.2(h)0(e)-275.2(d)0(a)16.5(t)12.2(a)-284(s)0(o)21.2(u)0(r)16.9(c)0(e)]TJ
0 -1.2266 TD
[(by)-343.9(me)21.5(an)16.1(s)-349.1(o)0(f)-344.7(t)12.2(he)-344.7()67.7()0(D)15.7(a)13.1(ta)-341.4(so)14.9(ur)23.2(ce)13.5()67.7()-349.6(bu)19.5(tt)18(on)-341.3(\(F)14.1(ig)23.7(.)-350.7(2)0(\))19(;)-350.7(si)20.4(nc)16.3(e)-354(i)0(n)-342(m)15.1(os)14.9(t)]TJ
0 -1.2203 TD
[(ex)16.9(pe)17.7(ri)21.1(me)21.5(nt)15.1(s)-228.9(n)0(o)19.1(t)-228.1(a)13.1(l)0(l)-215.7(g)0(e)14.7(n)0(e)22(s)-228.9(a)13.1(r)0(e)-221.9(t)12.2(es)17.8(te)18.6(d,)-220.9(t)12.2(h)0(e)-218.3(u)0(s)16.3(e)12.8(r)-228.3(c)0(a)20.2(n)-231(d)16(i)0(r)14.7(e)12.8(ct)19.2(ly)-216.6(en)15.7(t)12.2(e)0(r)]TJ
T*
[(th)15.1(e)-429.9(a)0(c)20.2(t)0(u)17.1(a)13.1(l)-433.5(l)0(i)18.2(s)0(t)-419.1(o)0(f)-420.6(g)0(e)14.7(n)0(e)22(s)-431.3(an)16.1(a)13.1(l)0(y)17.3(z)13.1(ed)-420.2(in)-424.2(a)-429.5(p)0(a)18.1(r)0(t)17.8(i)15.4(cu)18.3(la)15.9(r)-430.7(e)12.7(xp)15.5(e)12.7(r)0(i)21.1(m)0(e)15.2(n)0(t)]TJ
T*
[(\()73.5(T)14.1(e)12.7(s)0(t)17.2(e)12.8(d)-553.1(g)0(e)21(n)0(e)15.7(s)0()72.7()-552(op)21.2(ti)21.3(on)12.8(\))12.2(.)-553(In)-542.9(de)22.4(ta)19(il)18.2(,)-553(t)12.2(he)-540.7(da)16.5(t)12.2(a)-555.9(so)21.2(ur)16.9(c)13.4(e)-550(op)14.8(t)12.2(i)0(o)19(n)]TJ
0 -1.2266 TD
[()61.3()0(A)22(n)0(a)16.1(t)12.2(om)18.6(ic)16.2(a)13.1(l)-345(t)12.2(e)0(r)18.4(m)0(s)-334(o)0(n)-334.9(Z)0(F)23(I)0(N)-337.2(g)0(e)21(n)0(e)-332.1(s)0(u)16.3(m)0(m)17.5(a)13.1(ry)-333.9(pa)18.1(g)14.6(e)0(s)]TJ
/F8 1 Tf
22.2124 0 TD
(+)Tj
/F4 1 Tf
.7082 0 TD
[(st)23.5(ag)15(e)-341.3()0(l)18.8(-)]TJ
-22.9206 -1.2203 TD
[(te)18.6(r)73.3()-393.9(\()12.2(ab)21.4(br)20.2(ev)18.3(ia)15.9(t)12.2(e)0(d)-382.3(a)13.1(s)-393.3()67.7(g)19(en)15.7(e)-391.9(s)0(u)22.6(m)0(m)17.5(a)13.1(ry)20.2()61.3()0(\))-381.7(\()12.2(Fi)17.3(g.)-380.4(2)13.1(\))-392.5(us)16.3(e)12.8(s)-393.3(t)12.2(h)0(e)-382.7(a)13.1(na)16.1(-)]TJ
T*
[(to)15.7(mi)17.9(c)13.4(a)0(l)-900.9(t)0(e)18.6(r)0(m)14.4(s)-911.8(li)18.2(st)23.5(ed)-907.1(on)-904(Z)14.7(F)0(I)18.8(N)-916.8(g)14.6(e)0(n)15.7(e)-910.4(su)16.3(mm)17.5(a)13.1(r)0(y)-903(p)0(a)18.1(g)0(e)21(s)0(.)]TJ
0 -1.2267 TD
[(Fu)19.5(rt)17.8(he)15.7(rm)20.7(or)21.8(e,)-300(t)12.2(h)0(e)-300.5(u)0(s)22.6(e)0(r)-297.8(a)0(l)15.9(s)0(o)-294.9(h)0(a)16.1(s)-304.8(t)12.2(o)-306.3(se)17.8(le)15.5(c)13.4(t)-310.3(a)-303(s)0(t)17.2(a)13.1(ge)-301.5(\()12.2()61.3(S)18.6(t)12.2(a)0(g)21.4(e)-309.7(of)-294.1(de)16.1(-)]TJ
0 -1.2203 TD
[(ve)18.3(lo)19(pm)20.1(en)15.7(t)12.2()61.3(\))16.6(,)-432.9(w)0(h)20.3(i)0(c)22.5(h)-439.7(wi)20.1(ll)-424.4(b)14.6(e)-436.2(us)16.3(e)12.7(d)-433(fo)15.7(r)-430.7()0(l)18.8(t)0(e)18.6(r)0(i)14.7(n)0(g)-418.8(o)0(f)-426.9(a)0(n)16.1(a)13.1(to)15.7(m)15.1(i)0(c)16.2(a)13.1(l)]TJ
T*
[(te)18.6(rm)14.4(s)-292.2(k)0(n)17.5(o)0(w)20.9(n)-300.6(f)12.2(o)0(r)-288(t)0(h)15.1(e)-297.1(se)17.8(le)21.9(ct)19.2(ed)-287.4(st)17.2(a)13.1(g)0(e)-288.8(\()0(F)20.4(i)0(g)17.3(.)-293.8(1A)18.1(\).)-287.9(Th)18.9(e)-297.1(d)0(a)16.5(t)12.2(a)-296.7(s)0(o)14.9(u)0(r)23.2(c)0(e)]TJ
0 -1.2266 TD
[(op)14.8(t)12.2(i)0(o)19(n)-452.3()61.3()0(W)24.4(i)0(l)18.2(d)0(-)15.5(t)12.2(yp)19.5(e)-455.1(e)12.8(xp)15.5(re)18.4(ss)22.7(io)19(n:)-442.6(ma)21.9(nu)14.2(a)13.1(l)0(l)18.2(y)-453.3(a)13.1(n)0(n)18.5(o)0(t)15.7(a)13.1(te)18.6(d)-451.9(d)0(a)22.8(t)0(a)-442.6(f)0(o)15.7(r)]TJ
0 -1.2203 TD
[(so)14.9(me)-180.8(st)17.2(ag)21.4(es)17.8()61.3()-191.6(\(a)19(bb)16.5(re)24.7(vi)14.7(a)13.1(t)0(e)18.6(d)-192.7(as)-184.2()61.3()0(w)21.8(i)0(l)18.2(d)0(-)15.5(t)12.2(yp)19.5(e)-195.9(e)12.8(xp)15.5(re)18.4(ss)22.7(io)19(n)70.6(\))-185.7(is)-181.9(st)17.2(ri)21.1(ct)19.2(er)]TJ
T*
[(an)16.1(d)-344.4(w)0(i)20.1(l)0(l)-342.2(r)0(e)18.4(t)12.2(ri)21.1(ev)18.3(e)-347.7(t)0(h)15.1(o)0(s)21.2(e)-347.7(an)16.1(a)13.1(t)0(o)15.7(m)0(i)24.2(c)0(a)20.2(l)-351.3(t)12.2(e)0(r)18.4(m)0(s)-340.3(t)12.2(ha)16.1(t)-348.2(a)13.1(re)-342(e)12.7(x)0(p)15.5(e)12.7(ri)21.1(me)15.2(n-)]TJ
0 -1.2266 TD
[(ta)19(ll)18.2(y)-478.6(v)0(a)25(l)0(i)18.2(d)0(a)16.5(t)12.2(ed)-464.5(by)]TJ
/F5 1 Tf
8.4601 0 TD
[(in)-467.9(si)16.4(t)12.2(u)]TJ
/F4 1 Tf
3.2689 0 TD
[(hy)23.8(br)20.2(id)18.8(iz)15.9(a)13.1(t)0(i)21.3(o)0(n)-467.7(e)0(x)16.9(p)0(e)17.7(r)0(i)21.1(m)0(e)21.5(n)0(t)15.1(s)0(,)-465.8(a)13.1(s)-475.5(e)0(x)16.9(-)]TJ
-11.729 -1.2203 TD
[(pl)14.1(a)13.1(i)0(n)18.4(e)0(d)-394.9(p)0(r)23.2(e)0(v)18.3(i)0(o)19(u)0(s)16.3(l)0(y)-393.6(\()12.2(Fi)17.3(g)14.6(.)-407.6(1)13.1(B)0(\))15.5(.)-401.3(F)14.6(o)0(r)-395.5(t)0(h)15.1(i)0(s)-390.5(o)0(p)21.2(t)0(i)15(o)0(n)19.1(,)-401.3(th)15.1(e)-398.2(s)0(t)17.2(a)0(g)21.4(e)-404.6(l)18.8(t)12.2(e)0(r)]TJ
T*
[(op)14.8(t)12.2(i)0(o)19(n)-338.5(ha)22.4(s)-336.4(a)0(l)15.9(s)0(o)-326.6(b)0(e)21(e)0(n)-325.8(i)0(m)17.9(p)0(l)20.4(e)0(m)15.2(e)12.8(nt)15.1(e)12.8(d)0(.)-328.4(F)14.6(in)18.4(al)15.9(ly)23.7(,)-338(a)13.1(na)16.1(t)12.2(o)0(m)18.6(i)0(c)16.2(a)13.1(l)-338.6(t)12.2(er)18.4(ms)]TJ
T*
[(ar)18.8(e)-442.5(t)0(e)18.6(s)0(t)17.2(e)0(d)-432.9(f)0(o)15.7(r)-437(ov)15.5(e)12.7(r)0(-)17.8(r)0(e)18.4(p)0(r)16.9(e)12.8(se)17.8(nt)15.1(a)13.1(t)0(i)15(o)0(n)-429.8(u)0(s)16.3(i)0(n)18.4(g)-440.7(t)12.2(h)0(e)-433.2(h)0(y)17.5(p)0(e)17.7(r)0(g)20.2(e)0(o)16.3(m)15.1(et)18.6(ri)14.7(c)]TJ
0 -1.2266 TD
[(te)18.6(st)-235.7(wi)20.1(th)-237.8(Be)22.4(nj)17(a)13.1(m)0(i)17.9(n)0(i)18.4()13.1(Ho)16.2(c)13.4(h)0(b)17.5(e)0(r)18.4(g)-244.7(mu)20(lt)21.3(ip)20.4(le)-237.4(t)12.2(e)0(s)17.8(t)0(i)21.3(n)0(g)-235.4(c)0(o)16.9(r)0(r)23.9(e)0(c)13.5(t)12.2(io)19(n,)-233.9(a)13.1(n)0(d)]TJ
0 -1.2203 TD
[(th)15.1(e)-271.8(u)0(s)22.6(e)0(r)-259.8(c)0(a)13.9(n)-268.9(c)13.4(h)0(o)12.8(o)0(s)21.2(e)-271.8(ho)19.1(w)-267.2(t)0(o)-262.5(s)0(o)21.2(r)0(t)-266.7(t)12.2(he)15.7(m)-269.4(\()12.2()61.3()0(S)18.6(o)0(r)21.8(t)0(i)21.3(n)0(g)-260.7(o)0(p)14.8(t)12.2(io)19(n)70.6(\))16.6(.)]TJ
1.1128 -1.2203 TD
[(The)-183.3(input)-187.1(gene)-191(identiers)-183.1(are)-189.8(converted)-187.2(to)-186.6(ZFIN)-181.5(IDs,)-184.8(which)]TJ
-1.1128 -1.2267 TD
[(are)-284.6(used)-277.5(to)-281.4(query)-276.4(expression)-288.2(datasets)-283.7(stored)-280(on)-278(the)-282(server)-281.5(as)]TJ
28.0042 21.245 TD
[(at)-287.5(les.)-282.6(Next,)-290(the)-288.3(program)-285.2(obtains)-287.3(anatomical)-287.3(terms)-290.8(for)-288.5(the)]TJ
0 -1.2203 TD
[(in)18.4(pu)22.6(t)-234.4(g)0(e)21(n)0(e)-224.6(i)0(d)18.8(e)0(n)15.7(t)12.2(i)18.8(er)18.4(s)-235.3(f)12.2(ro)21.8(m)-237.8(e)12.7(it)21.3(he)15.7(r)-234.6(a)13.1(ll)-222(kn)17.5(ow)20.9(n)-237.3(z)13.1(eb)21(ra)18.8(s)14.7(h)-231(g)0(e)21(n)0(e)15.7(s)-228.9(or)]TJ
T*
[(f)12.2(r)0(o)15.5(m)-212.5(t)12.2(h)0(e)-205.6(i)0(n)18.4(p)0(u)16.3(t)-209.1(ge)14.7(ne)22(s)-216.3(p)0(r)16.9(o)0(v)21.8(i)0(d)18.8(e)12.7(d)-218(w)0(i)20.1(t)12.2(h)-218.4(t)12.2(he)-205.6()67.7()0(T)14.1(e)12.7(st)17.2(e)12.7(d)-218(ge)21(ne)15.7(s)79()-216.9(o)0(p)21.2(t)0(i)15(o)0(n)19.1(.)]TJ
0 -1.2266 TD
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T*
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T*
[(term:)-551.6(The)-556.4(selection)-557.9(of)-559.7(this)-552.5(option)-558.1(forces)-554.5(the)-560.2(program)-557.1(to)]TJ
T*
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[(ZEOGS)-336.3(ANALYSIS)-340.1(OF)-331.2(ANATOMICAL)-337.1(TERM)-336.8(ENRICHMENT)-336.8(IN)-335.1(GENE)-336.8(SETS)-17735.1(305)]TJ
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