Identification of Alternaria alternata mycotoxins by LC-SPE-NMR and their 
cytotoxic effects to soybean (Glycine max) cell suspension culture

de Souza, G. D.; Mithöfer, Axel; Daolio, C.; Schneider, Bernd; Rodrigues-Filho, E.

doi:10.3390/molecules18032528

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Identification of Alternaria alternata mycotoxins by LC-SPE-NMR and their cytotoxic effects to soybean (Glycine max) cell suspension culture

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Mithöfer, Axel
Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;

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Schneider, Bernd
Research Group Biosynthesis / NMR, MPI for Chemical Ecology, Max Planck Society;

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http://dx.doi.org/10.3390/molecules18032528
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BOL556.pdf
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Citation

de Souza, G. D., Mithöfer, A., Daolio, C., Schneider, B., & Rodrigues-Filho, E. (2013). Identification of Alternaria alternata mycotoxins by LC-SPE-NMR and their cytotoxic effects to soybean (Glycine max) cell suspension culture. Molecules, 18, 2528-2538. doi:10.3390/molecules18032528.

Cite as: https://hdl.handle.net/11858/00-001M-0000-000E-B0CD-C

Abstract

This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC50 values which range from 0.11 (±0.02) to 4.69 (±0.47) μM showed the high cytotoxicity of these compounds.