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Book Chapter

In Vivo Functional Imaging of the Olfactory Bulb at Single-Cell Resolution

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Direnberger,  Stephan
Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society;

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Griesbeck,  Oliver
Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society;

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Citation

Fink, S., Kovalchuk, Y., Homma, R., Schwendele, B., Direnberger, S., Cohen, L. B., et al. (2012). In Vivo Functional Imaging of the Olfactory Bulb at Single-Cell Resolution. In T. Fellin, & M. Halassa (Eds.), Neuromethods, Vol. 67 (pp. 21-43). Totowa: Humana Press. doi:10.1007/7657_2011_1.


Cite as: https://hdl.handle.net/11858/00-001M-0000-000E-AB68-2
Abstract
Functional properties of neuronal circuits can be best studied in vivo in the living mammalian brain. The use of optical methods, like two-photon calcium imaging, permits analyses of network function at single-cell resolution. This chapter provides a step-by-step description of this technique. Using mouse olfactory bulb as a model system, we compare the performance of genetically encoded calcium sensor TN-XXL and smallmolecule calcium indicators; describe how to choose the right calcium indicator and how to load it into the cells of interest; discuss the use of cell type-specific markers and, finally, illustrate the application of this technique for high-resolution in vivo imaging of sensory-driven neuronal activity