Quantification of protein-protein interactions within membranes by fluorescence 
correlation spectroscopy

Bleicken, Stephanie; Otsuki, Miki; García-Sáez, Ana Jesus

doi:10.2174/138920311798841744

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Quantification of protein-protein interactions within membranes by fluorescence correlation spectroscopy

MPS-Authors

/persons/resource/persons75297

Bleicken, Stephanie
Max Planck Research Group Membrane Biophysics, Max Planck Institute for Intelligent Systems, Max Planck Society;

/persons/resource/persons75924

Otsuki, Miki
Max Planck Research Group Membrane Biophysics, Max Planck Institute for Intelligent Systems, Max Planck Society;

/persons/resource/persons75485

García-Sáez, Ana Jesus
Max Planck Research Group Membrane Biophysics, Max Planck Institute for Intelligent Systems, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Bleicken, S., Otsuki, M., & García-Sáez, A. J. (2011). Quantification of protein-protein interactions within membranes by fluorescence correlation spectroscopy. Current Protein and Peptide Science, 12(8), 691-698. doi:10.2174/138920311798841744.

引用: https://hdl.handle.net/11858/00-001M-0000-0010-4F22-3

要旨

The characterization of interactions between membrane proteins as they take place within the lipid bilayer poses a technical challenge, which is currently very difficult and, in many cases, impossible to overcome. The recent development of a method based in the combination two-color fluorescence cross-correlation spectroscopy with scanning of the focal volume allows the detection and quantification of interactions between biomolecules inserted in biological membranes. This powerful strategy has allowed the quantitative analysis of diverse systems, such as the association between proteins of the Bcl-2 family involved in apoptosis regulation or the binding between a growth factor and its receptor during signaling. Here, we review the last developments to quantify protein/protein interactions in lipid membranes and focus on the use of fluorescence-correlation-spectroscopy approaches for that purpose.