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Modification of choline acetyltransferase by integration of green fluorescent protein does not affect enzyme activity and subcellular distribution

MPG-Autoren
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Gärtner,  A.
Emeritus Group: Neurochemistry / Thoenen, MPI of Neurobiology, Max Planck Society;

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Zitation

Rathenberg, J., Gärtner, A., Koenen, M., & Witzemann, V. (2002). Modification of choline acetyltransferase by integration of green fluorescent protein does not affect enzyme activity and subcellular distribution. Cell and Tissue Research, 308(1), 1-6.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0012-237C-0
Zusammenfassung
Choline acetyltransferase (ChAT) is widely used as a marker enzyme to identify cholinergic neurons in the central and peripheral nerve us system and to study developmental changes. In order to visualize expression of ChAT directly we have generated a ChAT-green fluorescent protein (GFP) fusion construct. Upon transfection of COS-1 cells and cultured rat hippocampal neurons, transgenic enzymatically active ChAT-GFP is expressed and shows intrinsic fluorescence. In COS-1 cells the ChAT-GFP construct revealed a subcellular distribution indistinguishable from wild-type ChAT. In primary neurons the fluorescence was present in the soma and neuritic processes. Hence, this construct will be useful for analyzing the expression and subcellular distribution of ChAT-GFP in cell and tissue culture.