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Journal Article

Lifeact: a versatile marker to visualize F-actin

MPS-Authors

Riedl, J.
Max Planck Society;

Crevenna, A. H.
Max Planck Society;

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Kessenbrock, K.
Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

Yu, J. H.
Max Planck Society;

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Neukirchen, D.
Max Planck Research Group: Axonal Growth and Regeneration / Bradke, MPI of Neurobiology, Max Planck Society;

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Bradke, F.
Max Planck Research Group: Axonal Growth and Regeneration / Bradke, MPI of Neurobiology, Max Planck Society;

/persons/resource/persons38911

Jenne, D.
Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

Holak, T. A.
Max Planck Society;

Sixt, M.
Max Planck Society;

Wedlich-Söldner, R.
Max Planck Society;

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Citation

Riedl, J., Crevenna, A. H., Kessenbrock, K., Yu, J. H., Neukirchen, D., Bista, M., et al. (2008). Lifeact: a versatile marker to visualize F-actin. Nature Methods, 5(7), 605-607.

Abstract

Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.