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Use of real-time PCR for determining copy number and zygosity in transgenic plants

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons3822

Bubner,  B.
Department of Molecular Ecology, MPI for Chemical Ecology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons3786

Baldwin,  I. T.
Department of Molecular Ecology, MPI for Chemical Ecology, Max Planck Society;

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Citation

Bubner, B., & Baldwin, I. T. (2004). Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Reports, 23(5), 263-271. doi:10.1007/s00299-004-0859-y.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0012-AC82-1
Abstract
This review examines how real-time PCR can be used to determine copy number and zygosity in transgenic plants. Distinguishing between plants that harbor one and two copies of a transgene or are hemizygous and homozygous requires the ability to routinely