de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues.

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons203920

Lukinavicius,  G.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons36443

Mitronova,  G.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons196937

Schnorrenberg,  S.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons191595

Butkevich,  A.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons219820

Barthel,  H.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons14832

Belov,  V. N.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons15210

Hell,  S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)

2567041.pdf
(Verlagsversion), 3MB

Ergänzendes Material (frei zugänglich)

2567041_Suppl.pdf
(Ergänzendes Material), 5MB

Zitation

Lukinavicius, G., Mitronova, G., Schnorrenberg, S., Butkevich, A., Barthel, H., Belov, V. N., et al. (2018). Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues. Chemical Science, 9(13), 3324-3334. doi:10.1039/C7SC05334G.


Zitierlink: http://hdl.handle.net/21.11116/0000-0000-FD55-4
Zusammenfassung
We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents – taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend on both components. We found correlation between the aggregation efficiency (related to the spirolactonization of fluorophore) and cytotoxicity. Probe optimization allowed us to reach 29 ± 11 nm resolution in stimulated emission depletion (STED) microscopy images of the microtubule network in living human fibroblasts. Application to living fruit fly (Drosophila melanogaster) tissues highlighted two distinct structures: microtubules and tracheoles. We identified 6-carboxy isomers of 580CP and SiR dyes as markers for chitin-containing taenidia, a component of tracheoles. STED microscopy revealed correlation between the taenidia periodicity and the diameter of the tracheole. Combined tubulin and taenidia STED imaging showed close interaction between the microtubules and respiratory networks in living tissues of the insect larvae.