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High-affinity functional fluorescent ligands for human β-adrenoceptors

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons36443

Mitronova,  G.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons203920

Lukinavicius,  G.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons191595

Butkevich,  A.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons14832

Belov,  V. N.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons15210

Hell,  S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Volltexte (frei zugänglich)

2483818.pdf
(Verlagsversion), 7MB

Ergänzendes Material (frei zugänglich)

2483818_Suppl.pdf
(Ergänzendes Material), 3MB

Zitation

Mitronova, G., Lukinavicius, G., Butkevich, A., Kohl, T., Belov, V. N., Lehnart, S. E., et al. (2017). High-affinity functional fluorescent ligands for human β-adrenoceptors. Scientific Reports, 7: 12319. doi:10.1038/s41598-017-12468-3.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-002D-FCD1-7
Zusammenfassung
Visualization of the G-protein coupled receptor (GPCR) is of great importance for studying its function in a native cell. We have synthesized a series of red-emitting fluorescent probes targeting β-adrenergic receptor (βAR) that are compatible with confocal and Stimulated Emission Depletion (STED) microscopy as well as with Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) binding assay in living cells. The probe based on the agonist BI-167107 and fluorescent dye KK114 demonstrates nanomolar binding affinity and up to nine-fold β2AR selectivity over β1AR. Carazolol-derived probes are fluorogenic and allow no-wash imaging experiments. STED microscopy of β2ARs stained at the native expression level on pancreatic CAPAN cells provides two-fold improvement in lateral optical resolution over confocal mode and reveals the formation of receptor microdomains. These probes retain their functional (agonist or antagonist) properties, allowing simultaneous modulation of cyclic adenosine monophosphate (cAMP) levels and receptor internalization as well as imaging receptor localization.