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Journal Article

SRpHi ratiometric pH biosensors for super-resolution microscopy.

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons136232

Richardson,  D. S.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons185783

Gregor,  C.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons205119

Winter,  F.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons39413

Urban,  N. T.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons15739

Sahl,  S. J.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons16030

Willig,  K. I.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons15210

Hell,  S. W.
Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society;

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Fulltext (public)

2481489.pdf
(Publisher version), 2MB

Supplementary Material (public)

2481489_Suppl.pdf
(Supplementary material), 554KB

Citation

Richardson, D. S., Gregor, C., Winter, F., Urban, N. T., Sahl, S. J., Willig, K. I., et al. (2017). SRpHi ratiometric pH biosensors for super-resolution microscopy. Nature Communications, 8: 577. doi:10.1038/s41467-017-00606-4.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002D-EEF9-F
Abstract
Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.