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Fluorescent photoswitchable diarylethenes for biolabeling and single molecule localization microscopies with optical superresolution.

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons199835

Roubinet,  B.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons205203

Weber,  M.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons204190

Shojaei,  H.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons36457

Bates,  M.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons14883

Bossi,  M. L.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons14832

Belov,  V. N.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons15210

Hell,  S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

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Zitation

Roubinet, B., Weber, M., Shojaei, H., Bates, M., Bossi, M. L., Belov, V. N., et al. (2017). Fluorescent photoswitchable diarylethenes for biolabeling and single molecule localization microscopies with optical superresolution. Journal of the American Chemical Society, 139(19), 6611-6620. doi:10.1021/jacs.7b00274.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-002D-3006-7
Zusammenfassung
A modular assembly of water soluble diarylethenes (DAEs), applicable as bio-markers for optical nanoscopy, is reported. Reversibly photoswitchable 1,2-bis(2-alkyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes possessing a fluorescent "closed" form were decorated with one or two methoxy group(s) attached to the para-position(s) of phenyl ring(s) and two, four or eight carboxylic acid groups. Antibody conjugates of these DAEs feature low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures and photophysical properties (high emission efficiencies and low cycloreversion quantum yields) enabling their application in superresolution microscopy. Images of tubulin, vimentin and nuclear pore complexes are presented. The superresolution images can also be acquired by using solely 488 nm light without additional photoactivation with UV light. These DAEs exhibit reversible photoswitching without requiring any additives to the imaging media and open new paths towards the modular design of fluorescent dyes for bioimaging with optical superresolution.