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Journal Article

Universal Super-Resolution Multiplexing by DNA Exchange

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons197583

Schueder,  Florian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons186093

Strauss,  Maximilian T.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons208763

Schnitzbauer,  Joerg
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons197581

Schlichthaerle,  Thomas
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons208765

Strauss,  Sebastian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons172959

Jungmann,  Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Schueder, F., Strauss, M. T., Hoerl, D., Schnitzbauer, J., Schlichthaerle, T., Strauss, S., et al. (2017). Universal Super-Resolution Multiplexing by DNA Exchange. Angewandte Chemie International Edition, 56(14), 4052-4055. doi:10.1002/anie.201611729.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002D-CE33-7
Abstract
Super-resolution microscopy allows optical imaging below the classical diffraction limit of light with currently up to 20 x higher spatial resolution. However, the detection of multiple targets (multiplexing) is still hard to implement and time-consuming to conduct. Here, we report a straightforward sequential multiplexing approach based on the fast exchange of DNA probes which enables efficient and rapid multiplexed target detection with common super-resolution techniques such as (d)STORM, STED, and SIM. We assay our approach using DNA origami nanostructures to quantitatively assess labeling, imaging, and washing efficiency. We furthermore demonstrate the applicability of our approach by imaging multiple protein targets in fixed cells.