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Journal Article

Effects of single-walled carbon nanotubes on the polymerase chain reaction

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons75375

Cui,  D.
Former Dept. Theory of Mesoscopic Phenomena, Max Planck Institute for Intelligent Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons76197

Tian,  F.
Former Dept. Theory of Mesoscopic Phenomena, Max Planck Institute for Intelligent Systems, Max Planck Society;
Dept. New Materials and Biosystems, Max Planck Institute for Intelligent Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons75701

Kong,  Y.
Former Dept. Theory of Mesoscopic Phenomena, Max Planck Institute for Intelligent Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons76201

Titushikin,  I.
Former Dept. Theory of Mesoscopic Phenomena, Max Planck Institute for Intelligent Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons75481

Gao,  H.
Former Dept. Theory of Mesoscopic Phenomena, Max Planck Institute for Intelligent Systems, Max Planck Society;

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Citation

Cui, D., Tian, F., Kong, Y., Titushikin, I., & Gao, H. (2003). Effects of single-walled carbon nanotubes on the polymerase chain reaction. Nanotechnology, 15(1), 154-157. doi:10.1088/0957-4484/15/1/030.


Abstract
The effects of single-walled carbon nanotubes on the polymerase chain reaction (PCR) were investigated via quantitative PCR product measurements, scanning electron microscopy (SEM), high-resolution transmission electron microscopy (TEM) and x-ray photoelectron spectroscopy (XPS). The results showed that adding single-walled carbon nanotubes (SWCNTs) into the reaction liquid increases the amount of PCR product at SWCNT concentrations below 3 µg µl−1, but this effect is reversed at higher SWCNT concentrations. Similar effects were observed in PCR reactions with or without Mg2+. Both SEM and HRTEM results showed that the DNA templates and Taq enzymes are attached to bundles of SWCNTs in PCR products. XPS results showed that the C 1s binding energy in PCR products increased after reaction, with the emergence of two new peaks beside the main peak compared with carbon nanotubes before reaction, suggesting that there might be a chemical reaction between SWCNTs and PCR components. In conclusion, SWCNTs may increase the PCR efficiency at a concentration range of less than 3 µg µl−1 in the reaction liquid and have the potential to act as catalysts in a variety of biochemical reactions.