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Molecular Basis for SMC Rod Formation and Its Dissolution upon DNA Binding

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons101905

Bürmann,  Frank
Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons145226

Toseland,  Christopher P.
Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons145229

Durand-Diebold,  Marie-Laure
Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons78036

Gruber,  Stephan
Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Soh, Y.-M., Bürmann, F., Shin, H.-C., Oda, T., Jin, K. S., Toseland, C. P., et al. (2015). Molecular Basis for SMC Rod Formation and Its Dissolution upon DNA Binding. MOLECULAR CELL, 57(2), 290-303. doi:10.1016/j.molcel.2014.11.023.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0025-73E3-4
Abstract
SMC condensin complexes are central modulators of chromosome superstructure in all branches of life. Their SMC subunits form a long intramolecular coiled coil, which connects a constitutive "hinge'' dimerization domain with an ATP-regulated "head'' dimerization module. Here, we address the structural arrangement of the long coiled coils in SMC complexes. We unequivocally show that prokaryotic Smc-ScpAB, eukaryotic condensin, and possibly also cohesin form rod-like structures, with their coiled coils being closely juxtaposed and accurately anchored to the hinge. Upon ATP-induced binding of DNA to the hinge, however, Smc switches to a more open configuration. Our data suggest that a long-distance structural transition is transmitted from the Smc head domains to regulate Smc-ScpAB's association with DNA. These findings uncover a conserved architectural theme in SMC complexes, provide a mechanistic basis for Smc's dynamic engagement with chromosomes, and offer a molecular explanation for defects in Cornelia de Lange syndrome.