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Miniaturization of two-photon microscopy for imaging in freely moving animals

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons93373

Helmchen,  Fritjof
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons128986

Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons93732

Kerr,  Jason
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

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Zitation

Helmchen, F., Denk, W., & Kerr, J. (2013). Miniaturization of two-photon microscopy for imaging in freely moving animals. Cold Spring Harbor Protocols, 2013(10), 904-913. doi:10.1101/pdb.top078147.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0024-C7A6-1
Zusammenfassung
This article describes the development and application of miniaturized two-photon-excited fluorescence microscopes (“two-photon fiberscopes”). Two-photon fiberscopes have been developed with the aim of enabling high-resolution imaging of neural activity in freely behaving animals. They use fiber optics to deliver laser light for two-photon excitation. Their small front piece typically contains a miniature scanning mechanism and imaging optics. Two-photon fiberscopes can be made sufficiently small and lightweight to be carried by rats and mice and to allow virtually unrestricted movement within a behavioral arena. Typically mounted to the animal’s skull above a cranial window, two-photon fiberscopes permit imaging of cells down to at least 250 µm below the brain surface (e.g., in rat neocortex). In freely exploring animals, action-potential-evoked calcium transients can be imaged in individual somata of visual cortex neurons bulk-labeled with a calcium indicator. Two-photon fiberscopes thus enable high-resolution optical recording of neural activity with cellular resolution during natural behaviors