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Global Proteome Turnover Analyses of the Yeasts S-cerevisiae and S-pombe

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons78437

Nagaraj,  Nagarjuna
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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1-s2.0-S2211124714009346-main.pdf
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Zitation

Christiano, R., Nagaraj, N., Froehlich, F., & Walther, T. C. (2014). Global Proteome Turnover Analyses of the Yeasts S-cerevisiae and S-pombe. CELL REPORTS, 9(5), 1959-1965. doi:10.1016/j.celrep.2014.10.065.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0024-A891-9
Zusammenfassung
How cells maintain specific levels of each protein and whether that control is evolutionarily conserved are key questions. Here, we report proteome-wide steady-state protein turnover rate measurements for the evolutionarily distant but ecologically similar yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe. We find that the half-life of most proteins is much longer than currently thought and determined to a large degree by protein synthesis and dilution due to cell division. However, we detect a significant subset of proteins (similar to 15%) in both yeasts that are turned over rapidly. In addition, the relative abundances of orthologous proteins between the two yeasts are highly conserved across the 400 million years of evolution. In contrast, their respective turnover rates differ considerably. Our data provide a high-confidence resource for studying protein degradation in common yeast model systems.