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Journal Article

Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene

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Koenen, Michael
Molecular anatomy of the neuromuscular junction, Max Planck Institute for Medical Research, Max Planck Society;
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Working Group Witzemann / Koenen, Max Planck Institute for Medical Research, Max Planck Society;
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Rüther, U., Koenen, M., Sippel, A. E., & Müller−Hill, B. (1982). Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene. Proceedings of the National Academy of Sciences of the USA (JSTOR), 79(22), 6852-6855. Retrieved from http://www.pnas.org/cgi/content/abstract/79/22/6852.

Abstract

A 10−kilobase DNA fragment containing exons 1 and 2 of the chicken lysozyme gene has been randomly cleaved with DNase I. After tailing and cloning into the plasmid pUK230, Lac+ colonies were selected. Colonies harboring expressed fragments of the exons could be detected by an immunoenzymatic assay using antibodies against lysozyme. The smallest fragment coded for 10 amino acids and the largest coded for almost all residues of exon 2. These results suggest that any gene of any genome cloned in this way can be detected if antibodies against the gene product are available