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Journal Article

Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene product and S−adenosylmethionine on the in vitro expression of the metB, metL and metJ genes

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons95345

Shoeman,  Robert L.
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Shoeman, R. L., Coleman, T., Redfield, B., Greene, R. C., Smith, A. A., Saint−Girons, I., et al. (1985). Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene product and S−adenosylmethionine on the in vitro expression of the metB, metL and metJ genes. Biochemical and Biophysical Research Communications, 133(2), 731-739. doi:10.1016/0006-291X(85)90965-9.


Abstract
The regulation of the expression of three Escherichia coli met genes, metB, which codes for cystathionine −synthetase (EC 4.2.99.9), metL, which codes for aspartokinase II−homoserine dehydrogenase II (EC 2.7.2.4−EC 1.1.1.3) and metJ, which codes for the methionine regulon aporepressor, has been studied using highly purified DNA−directed in vitro protein synthesis systems. In a system where the entire gene product is synthesized, the expression of the metB and metL genes is specifically inhibited by MetJ protein (repressor protein) and S−adenosylmethionine (AdoMet). In a simplified system that measures the formation of the first dipeptide of the gene product (fMet−Ala for the metJ gene), MetJ protein and AdoMet partially repress (˜40ÃÆ'¢â‚¬â€œ60%) metJ gene expression. Thus, the metJ gene can be partially autoregulated by its gene product