de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONS
  This item is discarded!DetailsSummary

Discarded

Journal Article

In vivo dendritic calcium dynamics in deep layer cortical pyramidal neurons

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons93373

Helmchen,  Fritjof
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;
In Vivo Microscopy of Cortical Dynamics, Max Planck Institute for Medical Research, Max Planck Society;
Cortical Two Photon Imaging, Max Planck Institute for Medical Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons128986

Denk,  Winfried
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Helmchen, F., Svoboda, K., Denk, W., & Tank, D. W. (1999). In vivo dendritic calcium dynamics in deep layer cortical pyramidal neurons. Nature Neuroscience, 2, 989-996. doi:10.1038/14788.


Abstract
Dendritic Ca2+ action potentials in neocortical pyramidal neurons have been characterized in brain slices, but their presence and role in the intact neocortex remain unclear. Here we used two−photon microscopy to demonstrate Ca2+ electrogenesis in apical dendrites of deep−layer pyramidal neurons of rat barrel cortex in vivo. During whisker stimulation, complex spikes recorded intracellularly from distal dendrites and sharp waves in the electrocorticogram were accompanied by large dendritic [Ca2+ ] transients; these also occurred during bursts of action potentials recorded from somata of identified layer 5 neurons. The amplitude of the [Ca 2+] transients was largest proximal to the main bifurcation, where sodium action potentials produced little Ca2+ influx. In some cases, synaptic stimulation evoked [Ca2+] transients without a concomitant action potential burst, suggesting variable coupling between dendrite and soma