de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Organization of focal adhesion plaques is disrupted by action of die HIV−1 protease

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons95345

Shoeman,  Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Shoeman, R. L., Hartig, R., Hauses, C., & Traub, P. (2002). Organization of focal adhesion plaques is disrupted by action of die HIV−1 protease. Cell Biology International, 26(6), 529-539. doi:10.1006/cbir.2002.0895.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0019-9F64-8
Zusammenfassung
Focal adhesion plaques were severely affected in human embryonic fibroblasts permeabilized with digitonin and incubated in buffer containing the human immunodeficiency virus type 1 protease (HIV−1 PR). A mutant HIV−1 PR (3271 HIV−1 PR) had no effect on focal adhesion plaques. Similar effects were seen with cells microinjected with either HIV−1 PR or 3271 HIV−1 PR. Immunoblots of the human embryonic fibroblasts demonstrated that a number of focal adhesion plaque proteins were specifically cleaved by HIV−1 PR. These included fimbrin, focal adhesion plaque kinase (FAK), talin, and, to a lesser extent, filamin, spectrin and fibronectin. Proteins detected by antibodies to 4 integrin and 3 integrin were also cleaved by the HIV−1 PR. Control experiments demonstrated that the effect and protein cleavages described are due to action of the HIV−1 PR and not to the action of endogenous host cell proteases