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Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons79592

Makino,  Debora Lika
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons77867

Conti,  Elena
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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ba5203.pdf
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Zitation

Makino, D. L., & Conti, E. (2013). Structure determination of an 11-subunit exosome in complex with RNA by molecular replacement. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 69, 2226-2235. doi:10.1107/S0907444913011438.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-C2CD-D
Zusammenfassung
The RNA exosome is an evolutionarily conserved multi-protein complex involved in the 3 degradation of a variety of RNA transcripts. In the nucleus, the exosome participates in the maturation of structured RNAs, in the surveillance of pre-mRNAs and in the decay of a variety of noncoding transcripts. In the cytoplasm, the exosome degrades mRNAs in constitutive and regulated turnover pathways. Several structures of subcomplexes of eukaryotic exosomes or related prokaryotic exosome-like complexes are known, but how the complete assembly is organized to fulfil processive RNA degradation has been unclear. An atomic snapshot of a Saccharomyces cerevisiae 420kDa exosome complex bound to an RNA substrate in the pre-cleavage state of a hydrolytic reaction has been determined. Here, the crystallographic steps towards the structural elucidation, which was carried out by molecular replacement, are presented.