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学術論文

Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate

MPS-Authors
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Martins,  Marina Camara Mattos
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Feil,  R.
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Krause,  Ursula
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Arrivault,  S.
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Vosloh,  Daniel
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Figueroa,  Carlos Maria
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Ivakov,  A.
Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Yadav,  Umesh Prasad
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Piques,  M.
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Metzner,  Daniela
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Stitt,  M.
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Lunn,  J. E.
System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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引用

Martins, M. C. M., Hejazi, M., Fettke, J., Steup, M., Feil, R., Krause, U., Arrivault, S., Vosloh, D., Figueroa, C. M., Ivakov, A., Yadav, U. P., Piques, M., Metzner, D., Stitt, M., & Lunn, J. E. (2013). Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate. Plant physiology, 163(3), 1142-1163. doi:10.1104/pp.113.226787.


引用: https://hdl.handle.net/11858/00-001M-0000-0014-A55E-D
要旨
Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (Arabidopsis thaliana) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by beta-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 m in the cytosol, 0.2 to 0.5 m in the chloroplasts, and 0.05 m in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night.