de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

NEW ENZYMATIC PROTECTING GROUP TECHNIQUES FOR THE CONSTRUCTION OF PEPTIDES AND GLYCOPEPTIDES

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons98735

Waldmann,  Herbert
Abt. IV: Chemische Biologie, Max Planck Institute of Molecular Physiology, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Waldmann, H., BRAUN, P., & KUNZ, H. (1991). NEW ENZYMATIC PROTECTING GROUP TECHNIQUES FOR THE CONSTRUCTION OF PEPTIDES AND GLYCOPEPTIDES. BIOMEDICA BIOCHIMICA ACTA, 50(10-11), S243-S248.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-9AD5-3
Zusammenfassung
The use of non-proteases for the selective removal of protecting groups from peptides and glycopeptides is described. The N-terminal deprotection of peptides can be achieved by the hyrolysis of the phenylacetyl (PhAc) amide blocking group catalyzed by penicillin G acylase. On the other hand, the lipase-mediated hydrolysis of n-heptyl (Hep) and 2-bromoethyl esters allows for the liberation of the C-terminal carboxy group. The selective C-terminal deprotection can be applied advantageously for the construction of acid- and base-sensitive polyfunctional O-glycopeptides. In all cases the enzymatic reactions are completely selective and proceed under mildest conditions (pH 7-8, r.t. to 37-degrees-C) without damaging the various other functionalities present in the complex substrates.