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Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons77715

Bauer,  Johann
Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society;

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Zitation

Pietsch, J., Ma, X., Wehland, M., Aleshcheva, G., Schwarzwaelder, A., Segerer, J., et al. (2013). Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission. BIOMATERIALS, 34(31), 7694-7705. doi:10.1016/j.biomaterials.2013.06.054.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-5BE3-7
Zusammenfassung
Human follicular thyroid cancer cells were cultured in Space to investigate the impact of microgravity on 3D growth. For this purpose, we designed and constructed a cell container that can endure enhanced physical forces, is connected to fluid storage chambers, performs media changes and cell harvesting automatically and supports cell viability. The container consists of a cell suspension chamber, two reserve tanks for medium and fixative and a pump for fluid exchange. The selected materials proved durable, non-cytotoxic, and did not inactivate RNAlater. This container was operated automatically during the unmanned Shenzhou-8 Space mission. FTC-133 human follicular thyroid cancer cells were cultured in Space for 10 days. Culture medium was exchanged after 5 days in Space and the cells were fixed after 10 days. The experiment revealed a scaffold-free formation of extraordinary large three-dimensional aggregates by thyroid cancer cells with altered expression of EGF and CTGF genes under real microgravity. (C) 2013 Elsevier Ltd. All rights reserved.