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Cryo-electron tomography: The challenge of doing structural biology in situ

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons78340

Lucic,  Vladan
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons78570

Rigort,  Alexander
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons77721

Baumeister,  Wolfgang
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Lucic, V., Rigort, A., & Baumeister, W. (2013). Cryo-electron tomography: The challenge of doing structural biology in situ. JOURNAL OF CELL BIOLOGY, 202(3), 407-419. doi:10.1083/jcb.201304193.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0014-4B3D-B
Abstract
Electron microscopy played a key role in establishing cell biology as a discipline, by producing fundamental insights into cellular organization and ultrastructure. Many seminal discoveries were made possible by the development of new sample preparation methods and imaging modalities. Recent technical advances include sample vitrification that faithfully preserves molecular structures, three-dimensional imaging by electron tomography, and improved image-processing methods. These new techniques have enabled the extraction of high fidelity structural information and are beginning to reveal the macromolecular organization of unperturbed cellular environments.