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NEW TYPE BIOSENSOR FOR THE DETECTION OF PESTICIDES, BASED ON THE INHIBITION OF ACETYLCHOLINESTERASE

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons101478

Nagy,  Peter
Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Zitation

Csiffary, G., Nagy, P., Kiss, A., & Adanyi, N. (2013). NEW TYPE BIOSENSOR FOR THE DETECTION OF PESTICIDES, BASED ON THE INHIBITION OF ACETYLCHOLINESTERASE. ACTA ALIMENTARIA, 42(1), 79-90. doi:10.1556/AAlim.42.2013.1.8.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-461F-8
Zusammenfassung
An amperometric biosensor for the detection of organophosphorus and carbamate type pesticides was developed. Acetylcholinesterase enzyme (electric eel) was immobilized in a thin-layer enzyme cell, and acetylthiocholine chloride (AcTCh) was used as substrate. Amperometric detection with glassy carbon electrode was performed in a stopped-flow FIA system. The inhibition effect of dichlorvos (DDVP), carbofuran, methomyl, and pirimicarb were examined: the linear measuring ranges were 0.001-0.1 mu mol l(-1), 0.001-0.1 mu mol l(-1), 0.2-1 mu mol l(-1), and 0.1-10 mu mol l(-1), respectively. Soil extract and apple juice were measured with spiking method. It was concluded that the biosensor can be used for screening pesticide residues in food and environmental samples.