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A functional analysis of the pyrimidine catabolic pathway in Arabidopsis

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons97498

Zrenner,  R.
Nucleotides and Sugars, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons97354

Riegler,  H.
Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;
Nucleotides and Sugars, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons97165

Geserick,  C.
Nucleotides and Sugars, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Zrenner, R., Riegler, H., Marquard, C. R., Lange, P. R., Geserick, C., Bartosz, C. E., et al. (2009). A functional analysis of the pyrimidine catabolic pathway in Arabidopsis. New Phytologist, 183(1), 117-132. doi:10.1111/j.1469-8137.2009.02843.x.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0014-2491-7
Abstract
Reductive catabolism of pyrimidine nucleotides occurs via a three-step pathway in which uracil is degraded to beta-alanine, CO2 and NH3 through sequential activities of dihydropyrimidine dehydrogenase (EC 1.3.1.2, PYD1), dihydropyrimidinase (EC 3.5.2.2, PYD2) and beta-ureidopropionase (EC 3.5.1.6, PYD3). A proposed function of this pathway, in addition to the maintenance of pyrimidine homeostasis, is the recycling of pyrimidine nitrogen to general nitrogen metabolism. PYD expression and catabolism of [2-C-14]-uracil are markedly elevated in response to nitrogen limitation in plants, which can utilize uracil as a nitrogen source. PYD1, PYD2 and PYD3 knockout mutants were used for functional analysis of this pathway in Arabidopsis. pyd mutants exhibited no obvious phenotype under optimal growing conditions. pyd2 and pyd3 mutants were unable to catabolize [2-C-14]-uracil or to grow on uracil as the sole nitrogen source. By contrast, catabolism of uracil was reduced by only 40% in pyd1 mutants, and pyd1 seedlings grew nearly as well as wild-type seedlings with a uracil nitrogen source. These results confirm PYD1 function and suggest the possible existence of another, as yet unknown, activity for uracil degradation to dihydrouracil in this plant. The localization of PYD-green fluorescent protein fusions in the plastid (PYD1), secretory system (PYD2) and cytosol (PYD3) suggests potentially complex metabolic regulation. New Phytologist (2009) 183: 117-132doi: 10.1111/j.1469-8137.2009.02843.x.