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Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons97430

Strenkert,  D.
Plant Molecular Chaperone Networks and Stress, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons97389

Schmollinger,  S.
Plant Molecular Chaperone Networks and Stress, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons97394

Schroda,  M.
Plant Molecular Chaperone Networks and Stress, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Strenkert, D., Schmollinger, S., & Schroda, M. (2011). Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii. Plant Methods, 7, 35. doi:10.1186/1746-4811-7-35.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0014-20B6-8
Abstract
We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR.