de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Optical Oxygen Micro- and Nanosensors for Plant Applications

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons97059

Ast,  C.
Energy Metabolism, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons97457

van Dongen,  J. T.
Energy Metabolism, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)

Ast-2012-Optical Oxygen Micro.pdf
(beliebiger Volltext), 345KB

Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Ast, C., Schmalzlin, E., Lohmannsroben, H. G., & van Dongen, J. T. (2012). Optical Oxygen Micro- and Nanosensors for Plant Applications. Sensors, 12(6), 7015-7032. doi:10.3390/S120607015.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-2056-D
Zusammenfassung
Pioneered by Clark's microelectrode more than half a century ago, there has been substantial interest in developing new, miniaturized optical methods to detect molecular oxygen inside cells. While extensively used for animal tissue measurements, applications of intracellular optical oxygen biosensors are still scarce in plant science. A critical aspect is the strong autofluorescence of the green plant tissue that interferes with optical signals of commonly used oxygen probes. A recently developed dual-frequency phase modulation technique can overcome this limitation, offering new perspectives for plant research. This review gives an overview on the latest optical sensing techniques and methods based on phosphorescence quenching in diverse tissues and discusses the potential pitfalls for applications in plants. The most promising oxygen sensitive probes are reviewed plus different oxygen sensing structures ranging from micro-optodes to soluble nanoparticles. Moreover, the applicability of using heterologously expressed oxygen binding proteins and fluorescent proteins to determine changes in the cellular oxygen concentration are discussed as potential non-invasive cellular oxygen reporters.