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Zeitschriftenartikel

Dynamics and segregation of cell-matrix adhesions in cultured fibroblasts

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons98742

Zamir,  E.
Abt. II: Systemische Zellbiologie, Max Planck Institute of Molecular Physiology, Max Planck Society;

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Zitation

Zamir, E., Katz, M., Posen, Y., Erez, N., Yamada, K. M., Katz, B. Z., et al. (2000). Dynamics and segregation of cell-matrix adhesions in cultured fibroblasts. Nature Cell Biology, 2(4), 191-196. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10783236.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-102D-A
Zusammenfassung
Here we use time-lapse microscopy to analyse cell-matrix adhesions in cells expressing one of two different cytoskeletal proteins, paxillin or tensin, tagged with green fluorescent protein (GFP). Use of GFP-paxillin to analyse focal contacts and GFP-tensin to study fibrillar adhesions reveals that both types of major adhesion are highly dynamic. Small focal contacts often translocate, by extending centripetally and contracting peripherally, at a mean rate of 19 micrometers per hour. Fibrillar adhesions arise from the medial ends of stationary focal contacts, contain alpha5beta1 integrin and tensin but not other focal-contact components, and associate with fibronectin fibrils. Fibrillar adhesions translocate centripetally at a mean rate of 18 micrometers per hour in an actomyosin-dependent manner. We propose a dynamic model for the regulation of cell-matrix adhesions and for transitions between focal contacts and fibrillar adhesions, with the ability of the matrix to deform functioning as a mechanical switch.