de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Konferenzbeitrag

Hepatocyte nuclear factor-4 (HNF-4α) is not involved in hypoxia induced Erythropoietin regulation in human neuroblastoma cell lines

MPG-Autoren

Berchner-Pfannschmidt,  Utta
Max Planck Institute of Molecular Physiology, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Stolze, I., Berchner-Pfannschmidt, U., Freitag, P., Frede, S., & Fandrey, J. (2002). Hepatocyte nuclear factor-4 (HNF-4α) is not involved in hypoxia induced Erythropoietin regulation in human neuroblastoma cell lines.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-0EC2-5
Zusammenfassung
Oxygen dependent Erythropoietin (EPO) production is regulated by hypoxia inducible factor-1 (HIF-1) which consists of the O2-labile α- and the constitutive β-subunit. In human hepatoma cells (HepG2) HNF-4α enhances hypoxia-induced EPO expression. Herein, we investigated EPO gene regulation in human neuroblastoma (NB) cell lines. Methods: EPO production in SH-SY5Y, Kelly and HepG2 cells was measured by real time PCR and EPO elisa. Intracellular distribution of HIF-1α, HIF-1β and HNF-4α was studied by Western blot and immunohistochemistry. Cells were transiently transfected with HIF-1-driven luciferase and a dominant negative (dn) HNF-4 plasmid. Results: Hypoxia induced EPO gene expression in NB cells was maximal at <0.1% O2. HIF-1α protein accumulated and translocated to the nucleus at an oxygen threshold of 7% O2 in HepG2 cells but at 3% O2 in NB. Differences were also seen in HIF-1α protein degradation upon reoxygenation. Very little HNF-4α was expressed in NB, but did not colocalize with HIF-1α under hypoxic conditions. Transfection with dnHNF-4 did not affect hypoxic induction of EPO in NB cells. Conclusions: HIF-1, but not HNF-4α is involved in EPO gene regulation in human NB cell lines. (This abstract is as it is in the printed issue of the journal).