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Single Molecule Recognition of Protein Binding Epitopes in Brush Border Membranes by Force Microscopy

MPG-Autoren

Wielert-Badt,  Susanne
Max Planck Institute of Molecular Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons98709

Lin,  Jiann-Trzuo
Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society;

Wimmer,  Barbara
Max Planck Institute of Molecular Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons98705

Kinne,  Rolf K. H.
Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society;

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Zitation

Wielert-Badt, S., Hinterdorfer, P., Gruber, H. J., Lin, J.-T., Badt, D., Wimmer, B., et al. (2002). Single Molecule Recognition of Protein Binding Epitopes in Brush Border Membranes by Force Microscopy. Biophysical Journal, 82(5): 1, pp. 2767-2774. Retrieved from http://www.biophysj.org/cgi/content/abstract/82/5/2767.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-0E82-8
Zusammenfassung
Sidedness and accessibility of protein epitopes in intact brush border membrane vesicles were analyzed by detecting single molecule interaction forces using molecular recognition force microscopy in aqueous physiological solutions. Frequent antibody-antigen recognition events were observed with a force microscopy tip carrying an antibody directed against the periplasmically located gamma-glutamyltrans- peptidase, suggesting a right side out orientation of the vesicles. Phlorizin attached to the tips bound to NA(+)/D-glucose cotransporter molecules present in the vesicles. The recognition was sodium dependent and inhibited by free phlorizin and D-glucose, and revealed an apparent K-D of 0.2 muM. Binding events were also observed with an antibody directed against the epitope aa603-aa630 close to the C terminus of the transporter. In the presence of phlorizin the probability of antibody binding was reduced but the most probable unbinding force f(u) = 100 pN remained unchanged. In the presence of D-glucose and sodium, however, both the binding probability and the most probable binding force (f(u) = 50 pN) were lower than in its absence. These studies demonstrate that molecular recognition force microscopy is a versatile tool to probe orientation and conformational changes of epitopes of membrane components during binding and traps-membrane transpor