de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Hypoxia-inducible erythropoietin gene expression in human neuroblastoma cells

MPG-Autoren

Berchner-Pfannschmidt,  Utta
Max Planck Institute of Molecular Physiology, Max Planck Society;

Wotzlaw,  Christoph
Max Planck Institute of Molecular Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons98672

Acker,  Helmut
Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Stolze, I., Berchner-Pfannschmidt, U., Freitag, P., Wotzlaw, C., Rössler, J., Frede, S., et al. (2002). Hypoxia-inducible erythropoietin gene expression in human neuroblastoma cells. Blood, 100(7): 1, pp. 2623-2628. Retrieved from http://dx.doi.org/10.1182/blood-2001-12-0169.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-0DF8-6
Zusammenfassung
Two human neuroblastoma (NB) cell lines, SH-SY5Y and Kelly, were found to express the gene for erythropoletin (EPO) in an oxygen (O-2)-dependent manner. However, NB cells had maximal production of EPO with lower partial pressure of O-2 values than the well-characterized hepatoma cell line HepG2. This maximal EPO expression was preceded by accumulation of the O-2- sensitive alpha subunit of the heterodimeric transcription- factor complex hypoxia-inducible factor 1 (HIF-1). Western blot analysis revealed that the amount of the beta subunit of HIF-1, identical to aryl hydrocarbon receptor nuclear translocator 1 (ARNT1), and the homolog ARNT2 increased in nuclear extracts from SH-SY5Y cells exposed to anoxia. In neuronal cells, ARNT1 and ARNT2 can form a heterodimer with HIF-1alpha, generating a functional HIF-1 complex. Using the hypoxia response element of the human EPO enhancer, we conducted electrophoretic mobility shift assays that showed accumulation and binding of HIF-1 complexes containing both ARNT1 and ARNT2 in NB cells. In addition to the HIF-1 complex, hepatocyte nuclear factor 4alpha (HNF4alpha) was found to be indispensable for hypoxia-induced EPO gene expression in hepatoma cells. Western blot analysis and polymerase chain reaction assessment showed that NB cells express neither HNF4alpha nor the splicing variant HNF4alpha7 and thus express EPO in an HNF4alpha-independent manner. Together, SH-SY5Y and Kelly cells may provide a new in vitro model for studying the mechanism of tissue-specific, hypoxia- inducible EPO gene expression. (C) 2002 by The American Society of Hematology.