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Zeitschriftenartikel

Pressure dependence of the photocycle kinetics of bacteriorhodopsin

MPG-Autoren

Klink,  Björn U.
Max Planck Institute of Molecular Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons98689

Engelhard,  Martin
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

Chizhov,  Igor
Max Planck Institute of Molecular Physiology, Max Planck Society;

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Zitation

Klink, B. U., Winter, R., Engelhard, M., & Chizhov, I. (2002). Pressure dependence of the photocycle kinetics of bacteriorhodopsin. Biophysical Journal, 83(6): 1, pp. 3490-3498.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0014-0DB1-6
Zusammenfassung
The pressure dependence of the photocycle kinetics of bacteriorhodopsin from <i>Halobacterium salinarium</i> was investigated at pressures up to 4 kbar at 25°C and 40°C. The kinetics can be adequately modeled by nine apparent rate constants, which are assigned to irreversible transitions of a single relaxation chain of nine kinetically distinguishable states <i>P</i>1 to <i>P</i>9. All states except <i>P</i>1 and <i>P</i>9 consist of two or more spectral components. The kinetic states <i>P</i>2 to <i>P</i>6 comprise only the two fast equilibrating spectral states L and M. From the pressure dependence, the volume differences ΔV0LM between these two spectral states could be determined that range from ΔV0LM = -11.4 ± 0.7 ml/mol (<i>P</i>2) to ΔV0LM = 14.6 ± 2.8 ml/mol (<i>P</i>6). A model is developed that explains the dependence of ΔV0LM on the kinetic state by the electrostriction effect of charges, which are formed and neutralized during the L/M transition.