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MAP2c, but not Tau, binds and bundles F-actin via its microtubule binding domain

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons98687

Dehmelt,  Leif
Abt. II: Systemische Zellbiologie, Max Planck Institute of Molecular Physiology, Max Planck Society;

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Roger, B., Al-Bassam, J., Dehmelt, L., Milligan, R. A., & Halpain, S. (2004). MAP2c, but not Tau, binds and bundles F-actin via its microtubule binding domain. Current Biology, 14(5): 1, pp. 363-371. Retrieved from http://dx.doi.org/10.1016/j.cub.2004.01.058.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0014-0B6C-5
Abstract
BACKGROUND: MAP2 and tau are abundant microtubule-associated proteins (MAPs) in neurons. The development of neuronal dendrites and axons requires a dynamic interaction between microtubules and actin filaments. MAPs represent good candidates to mediate such interactions. Although MAP2c and tau have similar, well-characterized microtubule binding activities, their actin interaction is poorly understood. RESULTS: Here, we show by using a cosedimentation assay that MAP2c binds F-actin. Upon actin binding, MAP2c organizes F-actin into closely packed actin bundles. Moreover, we show by using a deletion approach that MAP2c's microtubule binding domain (MTBD) is both necessary and sufficient for both F-actin binding and bundling activities. Surprisingly, even though the MAP2 and tau MTBDs share high sequence homology and possess similar microtubule binding activities, tau is unable to bind or bundle F-actin. Furthermore, experiments with chimeric proteins demonstrate that the actin binding activity fully correlates with the ability to promote neurite initiation in neuroblastoma cells. CONCLUSIONS: These results provide the first demonstration that the MAP2c and tau MTBD domains exhibit distinct properties, diverging in actin binding and neurite initiation activities. These results implicate a novel actin function for MAP2c in neuronal morphogenesis and furthermore suggest that actin interactions could contribute to functional differences between MAP2 and tau in neurons.