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Membrane Fusion Intermediates via Directional and Full Assembly of the SNARE Complex

MPG-Autoren

Hernandez,  Javier M.
Max Planck Institute of Molecular Physiology, Max Planck Society;

Stein,  Alexander
Max Planck Institute of Molecular Physiology, Max Planck Society;

Behrmann,  Elmar
Max Planck Institute of Molecular Physiology, Max Planck Society;

Riedel,  Dietmar
Max Planck Institute of Molecular Physiology, Max Planck Society;

Cypionka,  Anna
Max Planck Institute of Molecular Physiology, Max Planck Society;

Farsi,  Zohreh
Max Planck Institute of Molecular Physiology, Max Planck Society;

Walla,  Peter J.
Max Planck Institute of Molecular Physiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons135422

Raunser,  Stefan
Abt. III: Strukturbiochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;

Jahn,  Reinhard
Max Planck Institute of Molecular Physiology, Max Planck Society;

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Zitation

Hernandez, J. M., Stein, A., Behrmann, E., Riedel, D., Cypionka, A., Farsi, Z., et al. (2012). Membrane Fusion Intermediates via Directional and Full Assembly of the SNARE Complex. Science, 336(6088): 1, pp. 1581-1584. Retrieved from http://dx.doi.org/10.1126/science.1221976.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0013-FED3-B
Zusammenfassung
Cellular membrane fusion is thought to proceed through intermediates including docking of apposed lipid bilayers, merging of proximal leaflets to form a hemifusion diaphragm, and fusion pore opening. A membrane-bridging four-helix complex of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediates fusion. However, how assembly of the SNARE complex generates docking and other fusion intermediates is unknown. Using a cell-free reaction, we identified intermediates visually and then arrested the SNARE fusion machinery when fusion was about to begin. Partial and directional assembly of SNAREs tightly docked bilayers, but efficient fusion and an extended form of hemifusion required assembly beyond the core complex to the membrane-connecting linkers. We propose that straining of lipids at the edges of an extended docking zone initiates fusion.