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Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons39002

Mues,  Marsilius
Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons38757

Bartholomäus,  Ingo
Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons59463

Thestrup,  Thomas
Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons38863

Griesbeck,  Oliver
Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons39114

Wekerle,  Hartmut
Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons38918

Kawakami,  Naoto
Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons38946

Krishnamoorthy,  Gurumoorthy
Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society;

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Citation

Mues, M., Bartholomäus, I., Thestrup, T., Griesbeck, O., Wekerle, H., Kawakami, N., et al. (2013). Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator. NATURE MEDICINE, 19(6), 778-783. doi:10.1038/nm.3180.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-FB73-E
Abstract
To study T cell activation in vivo in real time, we introduced a newly developed fluorescence resonance energy transfer-based, genetically encoded calcium indicator into autoantigen-specific and non-autoantigen-specific CD4(+) T cells. Using two-photon microscopy, we explored the responses of retrovirally transduced calcium indicator-expressing T cells to antigen in the lymph nodes and the central nervous system. In lymph nodes, the administration of exogenous antigen caused an almost immediate arrest of T cells around antigen-presenting cells and an instant rise of cytosolic calcium. In contrast, encephalitogenic T cells entering the leptomeningeal space, one main portal into the central nervous system parenchyma during experimental autoimmune encephalomyelitis, showed elevated intracellular calcium concentrations while still meandering through the space. This approach enabled us to follow the migration and activation patterns of T cells in vivo during the course of the disease.