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Membrane and actin reorganization in electropulse-induced cell fusion

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons78003

Gerisch,  Günther
Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons77922

Ecke,  Mary
Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons98202

Neujahr,  Ralph
Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons78526

Prassler,  Jana
Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons98204

Stengl,  Andreas
Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Gerisch, G., Ecke, M., Neujahr, R., Prassler, J., Stengl, A., Hoffmann, M., et al. (2013). Membrane and actin reorganization in electropulse-induced cell fusion. JOURNAL OF CELL SCIENCE, 126(9), 2069-2078. doi:10.1242/jcs.124073.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-F663-3
Abstract
When cells of Dictyostelium discoideum are exposed to electric pulses they are induced to fuse, yielding motile polykaryotic cells. By combining electron microscopy and direct recording of fluorescent cells, we have studied the emergence of fusion pores in the membranes and the localization of actin to the cell cortex. In response to electric pulsing, the plasma membranes of two contiguous cells are turned into tangles of highly bent and interdigitated membranes. Live-imaging of cells double-labeled for membranes and filamentous actin revealed that actin is induced to polymerize in the fusion zone to temporarily bridge the gaps in the vesiculating membrane. The diffusion of green fluorescent protein (GFP) from one fusion partner to the other was scored using spinning disc confocal microscopy. Fusion pores that allowed intercellular exchange of GFP were formed after a delay, which lasted up to 24 seconds after exposure of the cells to the electric field. These data indicate that the membranes persist in a fusogenic state before pores of about 3 nm diameter are formed.