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Journal Article

Endothelin-1 increases serum-glucocorticoid-regulated kinase-1 expression in smooth muscle cells.

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons84200

Schultze,  M
Department Human Perception, Cognition and Action, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Citation

Wolf, S., Schultze, M., Sauter G, Risler, T., & Brehm, B. (2004). Endothelin-1 increases serum-glucocorticoid-regulated kinase-1 expression in smooth muscle cells. Journal of Cardiovascular Pharmacology, 44(Supplement 1), S304-S306. Retrieved from http://pdfs.journals.lww.com/cardiovascularpharm/2004/11001/Endothelin_1_Increases.75.pdf?token=method|ExpireAbsolute;source|Journals;ttl|1266847590242;payload|mY8D3u1TCCsNvP5E421JYPPlNl9ZUXrQDsjmMHeXqBgfxP56d5BAis+WhfSrPR1S6lcHrAT5WTvTkrI7Jc1zUq2UlEn8N1x.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-D75F-9
Abstract
The mechanism of salt-sensitive hypertension is not fully understood. Several studies point to a possible role of endothelin (ET)-1 in this form of hypertension. Serum-regulated and glucocorticoid-regulated kinase-1 (SGK1) mediates trafficking of the renal epithelial sodium channel. The aim of the study was to find out whether ET-1 regulates SGK1. Rat smooth muscle cells were incubated with ET-1 (10(-7) M, 0-120 minutes). After 30 minutes a significant increase in SGK1 mRNA was found (122 +/- 4.2), and a maximum was reached after 120 minutes (217 +/- 7.6). Incubation of smooth muscle cells with ET-1 (10(-7) mol/L) in the presence of an ETA receptor antagonist inhibited SGK1 gene transcription (93 +/- 3.7). Western blot analysis showed a time-dependent increase in SGK1 protein in smooth muscle cells. These data indicate that ET-1 increases SKG1 mRNA and protein concentration. Inhibition of ET-1 by ET antagonism prevented a SGK1 increase. Therefore, ET antagonism might influence blood pressure by regulating the sodium balance through reducing SGK1 gene expression.