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Journal Article

Simultaneous determination of five bioactive flavonoids in Hypericum japonicum thunb by Reversed-Phase Liquid Chromatography.

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons84059

Li,  J
Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Citation

Li, J., Jiang B, Liu X, Zhang J, Chen, H., & Bi, S. (2007). Simultaneous determination of five bioactive flavonoids in Hypericum japonicum thunb by Reversed-Phase Liquid Chromatography. Asian Journal of Traditional medicines., 2(2), 75-81. Retrieved from http://www.asianjtm.com/qikan/manage/wenzhang/AJTM07-2(2)-7.pdf.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-CEAD-2
Abstract
A simple reversed-phase liquid chromatographic method has been established for the simultaneous quantification of five bioactive flavonoids, i.e., taxifolin-7-O-rhamnoside, isoquercitrin, quercitrin, quercetin and kaempferol in Hypericum japonicum Thunb. Chromatographic separations were achieved on a Luna C18 column (250 mm×4.6 mm, 5 amp;956;m) with a gradient of methanol and 0.5 aqueous acetic acid (v/v) as mobile phase and UV detection at 350 nm. The assay was reproducible with overall intra- and inter-day variation of less than 4.8 . The mean recovery of the method was 99.8 ± 2.5 . Using the optimized method, 19 samples were analyzed. The results indicated that the developed HPLC assay could be readily utilized as a quality control method for Hypericum japonicum Thunb.