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MR Contrast Agent Coupled to an Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons83903

Engelmann,  J
Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons84244

Su,  W
Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons84085

Mishra,  R
Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons84137

Pfeuffer,  J
Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Citation

Engelmann, J., Su, W., Mishra, R., Pfeuffer, J., Wiesmüller, K., & Ugurbil, K. (2007). MR Contrast Agent Coupled to an Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate. Poster presented at 2007 Joint Annual Meeting ISMRM-ESMRMB, Berlin, Germany.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-CDE1-1
Abstract
An antisense MR contrast agent and its nonsense counterpart, conjugated to a peptide nucleic acid and a cell penetrating peptide were synthesized capable of targeting cellular mRNA. Intracellular uptake in non-targeted 3T3 fibroblasts was confirmed by fluorescence and MR studies. The intracellular relaxation rate R1,cell increased significantly already at a labeling concentration of 0.5 μM, thus contrast enhancement was also detectable. An in vitro binding assay provided first evidence of a higher specificity of the antisense contrast agent. These results demonstrate its potential to be used as a targeted contrast agent for tracking mRNA transcription in cells by MRI.