de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Poster

MR Contrast Agent Coupled to an Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons83903

Engelmann,  J
Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons84244

Su,  W
Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons84085

Mishra,  R
Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons84137

Pfeuffer,  J
Department Physiology of Cognitive Processes, Max Planck Institute for Biological Cybernetics, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Engelmann, J., Su, W., Mishra, R., Pfeuffer, J., Wiesmüller, K., & Ugurbil, K. (2007). MR Contrast Agent Coupled to an Antisense Peptide Nucleic Acid - Cell Penetrating Peptide Conjugate. Poster presented at 2007 Joint Annual Meeting ISMRM-ESMRMB, Berlin, Germany.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0013-CDE1-1
Zusammenfassung
An antisense MR contrast agent and its nonsense counterpart, conjugated to a peptide nucleic acid and a cell penetrating peptide were synthesized capable of targeting cellular mRNA. Intracellular uptake in non-targeted 3T3 fibroblasts was confirmed by fluorescence and MR studies. The intracellular relaxation rate R1,cell increased significantly already at a labeling concentration of 0.5 μM, thus contrast enhancement was also detectable. An in vitro binding assay provided first evidence of a higher specificity of the antisense contrast agent. These results demonstrate its potential to be used as a targeted contrast agent for tracking mRNA transcription in cells by MRI.