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Visually evoked activity in cortical cells imaged in freely moving animals

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons84186

Sawinski,  J
Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons84296

Wallace,  DJ
Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons83945

Greenberg,  DS
Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons84010

Kerr,  JND
Research Group Neural Population Imaging, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Zitation

Sawinski, J., Wallace, D., Greenberg, D., Grossmann S, Denk, W., & Kerr, J. (2009). Visually evoked activity in cortical cells imaged in freely moving animals. Proceedings of the National Academy of Sciences of the United States of America, 106(46), 19557-19562. doi:10.1073/pnas.0903680106.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0013-C214-9
Zusammenfassung
We describe a miniaturized head-mounted multiphoton microscope and its use for recording Ca2+ transients from the somata of layer 2/3 neurons in the visual cortex of awake, freely moving rats. Images contained up to 20 neurons and were stable enough to record continuously for >5 min per trial and 20 trials per imaging session, even as the animal was running at velocities of up to 0.6 m/s. Neuronal Ca2+ transients were readily detected, and responses to various static visual stimuli were observed during free movement on a running track. Neuronal activity was sparse and increased when the animal swept its gaze across a visual stimulus. Neurons showing preferential activation by specific stimuli were observed in freely moving animals. These results demonstrate that the multiphoton fiberscope is suitable for functional imaging in awake and freely moving animals.