de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Disclaimer Contact us Login
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Conference Paper

Glutamine-free media for vaccine production processes

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons86303

Genzel,  Y.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86448

Reichl,  U.
Otto-von-Guericke-Universität Magdeburg;
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Genzel, Y., Alt, R., & Reichl, U. (2005). Glutamine-free media for vaccine production processes. In F. Godia, & M. Fussenegger (Eds.), Animal Cell Technology meets Genomics: Proceedings ot the 18th ESACT Meeting (pp. 767-770).


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-A04A-0
Abstract
In cell culture technology high ammonia concentrations can limit cell growth and product formation, especially when focussing on higher cell densities. This high ammonia concentration normally results from glutamine consumption and decomposition. To overcome such problems we have tested several options and developed a glutamine-free medium for cell lines used in industrial applications. Preliminary investigations were carried out in static cultures of BHK 21, CHO and MDCK cells. Detailed characterization of MDCK cell metabolism was done for a vaccine production process of influenza virus (equine influenza Newmarket 1/93 H3N8) in MDCK cells using this newly developed glutamine-free medium: The results for cell growth and virus replication in a large-scale controlled microcarrier culture (5L bioreactor) are compared to the production in glutamine containing (2 mM) GMEM medium. Amino acid uptake and release will be compared with other on- & off-line data, to evaluate the importance of some amino acids in different phases of cell metabolism and virus replication with respect to lactate and ammonia production. © 2005 Springer. Printed in the Netherlands [accessed 2013 November 18th]