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Downstream Processes and Glycan Characterization of MDCK-Cell Derived Influenza Virus

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons86517

Wolff,  M. W.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;
Otto-von-Guericke-Universität Magdeburg, External Organizations;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86343

Kalbfuss,  B.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86386

Lehmann,  S.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86422

Opitz,  L.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86531

Zimmermann,  A.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86448

Reichl,  U.
Otto-von-Guericke-Universität Magdeburg;
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Zitation

Wolff, M. W., Kalbfuss, B., Lehmann, S., Morenweiser, R., Opitz, L., Zimmermann, A., et al. (2006). Downstream Processes and Glycan Characterization of MDCK-Cell Derived Influenza Virus. Talk presented at BioProcess Technology - Asia. Singapore. 2006-06-26 - 2006-06-28.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0013-9A21-E
Zusammenfassung
Due to its annual death rate and the potential to cause pandemics, Influenza remains a major public health concern. Efforts to control the annual spread of influenza have centered on prophylactic vaccinations. Influenza viruses for human vaccine production are currently grown on embryonated eggs. However, this production process conveys major drawbacks such as lack of scalability, inability of some strains to replicate on eggs to high enough yields, and possible allergic reactions induced by egg proteins. These limitations emphasize the need for an alternative process. In recent years several continuous cell lines such as the Madin-Darby canine kidney (MDCK) have been successfully established for the production of influenza vaccines. These processes require the modification of existing downstream strategies to account for the modified upstream technology. The presented study focuses on the development of two downstream processing schemes for the purification of human Influenza A/PR/8/34 (H1N1) virus. Purification scheme 1 is based on a combination of size exclusion and anion exchange chromatography. Scheme 2 is centered on a lectin affinity chromatography. Both downstream processes represent a promising tool for the effective processing of MDCK-cell derived vaccines. In addition, the glycan analysis provides a powerful tool for the quality control of cell and egg derived antigens.